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Liverpool Women's NHS Foundation Trust  
Clinical Services next level down   Genetics Cytogenetic Service Samples  
Liverpool Women's Hospital
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  Crown Street
Liverpool, L8 7SS
Tel: tel:0151 708 9988
Fax: 0151 702 4028		 curve

Sample Specifications
   
If you are requesting: Please send:

Prenatal Diagnosis by:

  
  • Amniocentesis
 15-20 ml Amniotic Fluid in a sterile plastic container

  • Chorionic Villus Sampling
  • Cytogenetics Only - 5-10 mg Villus tissue in culture media

  • Cytogenetics & Molecular - 15mg Villus tissue in culture media

  • Biochemical referrals - >20 mg Villus tissue in culture media

    (N.B. Culture media is available from the Cytogenetics Laboratory).
    		•

    • Fetal Blood


    1-2 ml of blood in a lithium heparin container.

     

    Peripheral Blood chromosome
    Analysis

     
  • Child 1-5 ml blood in lithium heparin container, or
  • Adult 5-10 ml blood in lithium heparin container
    		•

    Bone Marrow leukaemia analysis


    1-2 ml of aspirated bone marrow in culture media (available from the Cytogenetics Laboratory).

     

    Solid Tissue (Fetal, Placenta or RPC) culture


    Solid tissue samples must be sent in transport medium (available from the Cytogenetics laboratory). A needle punch biopsy is sufficient from live patients.
    The table below gives sampling details for fetal referrals.

    Referral Type Tissue Required
    Stillborn / IUD (within 24hrs of event).At least one of:
  • Cord blood in lithium heparin
  • Cardiac Blood in lithium heparin
  • Full-depth skin biopsy and muscle
    PLUS
    Placental tissue from cord insertion site with membranes attached
    		•
    Stillborn / IUD (> 24hrs of event). Placental tissue from Cord insertion site with membranes attached
    PLUS Full-depth skin & muscle biopsy, if possible.
    TOP for fetal abnormality on Ultrasound scan a) 10-20ml Amniotic Fluid.
    b) Placental tissue as above.
    c) Cord Blood in lithium heparin.
    d) Cardiac Blood in lithium heparin.
    e) Full-depth Skin & Muscle biopsy.
    N.B.The above solid tissue samples should be at least (1cm3) in transport medium.
     

    Tumour Culture


    Representative tissue in transport media
    (available from the Cytogenetics laboratory).

     

    Fluorescent Insitu Hybridisation
    Studies


    Samples as for the relevant tissue above.
    Please indicate on the referral card which tests are required. (Availability).

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    Page revised 17.06.04 (Alan Clark)
    Authorised 23.06.04 (Peter Howard)